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上海拜力生物科技公司
Tel:400-968-7988    021-33779008
Anti-Histone H3.3 Rabbit Monoclonal Antibody, Clone RM190
品牌：RevMAb
貨號：31-1058-00
規(guī)格：100 ug
貨期：電詢
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Product Details
Product Name Anti-Histone H3.3 Rabbit Monoclonal Antibody [RM190]
Product Description Rabbit monoclonal to Histone H3.3
Catalog No. 31-1058-00
Clone Name RM190
Product Data Sheet Anti-Histone H3.3 Rabbit Monoclonal Antibody Data Sheet RM190
Specificity RM190 reacts to Histone H3.3, independent of post-translational modifications. No cross reactivity with Histone H3.1 or other histone proteins.
Immunogen A peptide corresponding to human Histone H3.3.
Application Western Blot (WB)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.5 ug/mL – 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.05 ug/mL – 0.5 ug/mL.
Keywords
References for Histone H3.3 Rabbit Monoclonal Antibody [RM190] Hinchcliffe EH et al. Chromosome missegregation during anaphase triggers p53 cell cycle arrest through histone H3.3 Ser31 phosphorylation, Nat Cell Biol., 10.1038/ncb3348. (2016) IF; Human, Monkey. Read more (PubMed: 27136267)
Product Images
RM190

RM190 reacts specifically to Histone H3.3.
No cross reactivity with Histone H3.1.

Western

Western Blot of HeLa whole cell lysate,
recombinant Histone H3.3 and Histone H3.1
 proteins, using RM190 at 1 ug/mL, showed
 a band of histone H3.3 in HeLa cells and
 recombinant H3.3 protein, and no cross
 activity with Histone H3.1.


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