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Anti-Phospho-Histone H3 (Thr3) Rabbit Monoclonal Antibody, Clone RM159; H3pThr3, Histone H3 (Thr3 phosphorylation)
品牌:RevMAb
貨號(hào):31-1072-00
規(guī)格:100 ug
貨期:電詢
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Product Details
Product Name Anti-Phospho-Histone H3 (Thr3) Rabbit Monoclonal Antibody [RM159]
Product Description Rabbit monoclonal to Phospho-Histone H3 (Thr3), Histone H3 (Thr3 phosphorylation)
Catalog No. 31-1072-00
Clone Name RM159
Product Data Sheet Anti-Phospho-Histone H3 (Thr3) Rabbit Monoclonal Antibody Data Sheet RM159
Specificity RM159 reacts to Histone H3 phosphorylated at Threonine 3. No cross reactivity with other phosphorylated histones
Immunogen A phospho-peptide corresponding to Phospho-Histone H3 (Thr3)
Application Western Blot (WB)
Immunocytochemistry (ICC)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.1 ug/mL - 1 ug/mL;
ICC: 0.5 ug/mL - 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 1 ug/mL.

Keywords
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Product Images

Western Blot of acid extracts of HeLa cells non-treated
(1) or treated (2) with Nocodazole, and recombinant
Histone H3.3 (3). Using RM159 at 0.1 ug/mL, showed
a band of Histone H3 phosphorylated at threonine 3
in HeLa cells
.

Immunocytochemistry

Immunocytochemistry of HeLa cells using Phospho-Histone H3
(Thr3) Rabbit mAb RM159 (red). Actin filaments have been
labeled with fluorescein phalloidin (green).

Immunocytochemistry

Immunocytochemistry of HeLa cells using Phospho-Histone H3
(Thr3) Rabbit mAb RM159 (red). Actin filaments have been
labeled with fluorescein phalloidin (green).


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