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Anti-Mouse IgG Rabbit Monoclonal Antibody, Clone RM104

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Tel:400-968-7988 021-33779008

Anti-Mouse IgG Rabbit Monoclonal Antibody, Clone RM104

品牌：RevMAb

貨號：31-1001-00

規(guī)格：100ug

貨期：電詢

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Product Details
Product Name	Anti-Mouse IgG Rabbit Monoclonal Antibody [RM104]
Product Description	Rabbit monoclonal to Mouse IgG
Catalog No.	31-1001-00
Clone Name	RM104
Product Data Sheet	Anti-Mouse IgG Rabbit Monoclonal Antibody Data Sheet RM104
Specificity	RM104 reacts to the Fc region of all mouse IgG subclasses. No cross reactivity with mouse IgM, IgA, IgE, human IgG, and rat IgG. It may cross react to goat IgG. The Fc region of RM104 has been engineered to eliminate Fc receptor binding.
Immunogen	Mouse IgG
Application	Western Blot (WB) (nonreduced) Immunoprecipitation (IP) Immunohistochemistry (IHC) Flow Cytometry (FC) Immunocytochemistry (ICC) ELISA
Species Reactivity	Mouse
Conjugate	None
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size	100ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	ELISA: 0.005 ug/mL-0.2 ug/mL; Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL; Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL; Western Blot (WB): 0.1 ug/mL-0.5 ug/mL.
Keywords
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Product Images
	Western blot of nonreduced(-) and reduced(+)mouse immunoglobulins (20ng/lane), using 0.2ug/mL of RevMAb clone RM104. This antibodyreacts to nonreduced mouse IgG1, IgG2a, IgG2b,and IgG3. It showed no cross reactivity with IgM, IgA, or IgE.
	ELISA of mouse immunoglobulins shows RM104reacts to the Fc region of mouse IgG1, IgG2a,IgG2b, and IgG3; no cross reactivity with IgM,IgA, IgE, human or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins.200 ng/mL, 50 ng/mL, or 10 ng/mL of RM104 was used as the primary antibody. An alkaline phosphataseconjugated anti-rabbit IgG as the secondary antibody.
	A titer ELISA of mouse IgG. The plate was coatedwith different amounts of mouse IgG. A serial dilutionof RM104 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.